Tag Archives: Coriolus versicolor

Diabetes, Personal Testimonies

How inForce immune builder saved my foot from being amputated due to diabetes.

I always knew that diabetes ran in my family, starting with my Dad (William T. Kaufman) who past away from type one diabetes. My brother Jerry was diagnosed with type 2 diabetes about 10 years a go and my other brother Bill was type one diagnoses. My twin sister Paulette is also type 2.

Early 2010 I had a job that required travel and I had recently returned from one of my long trips. I went to the cleaners to wash my close and while standing at the counter I blacked out and they thought I had a heart attack. When I came through I thought it was just jet lag and fatigue. I then went and got something to eat and drink and did not think of it as being diabetic.

Around mid July 2010 I was strolling on the beach with a friend when I got a splinter in the palm of my foot. I was very fatigue that day so I went home and took a three hour nap and when I woke I had a temp of 108 and my foot was swelling up and would not go down. Two or three days later I decided to go to the emergency because of the redness and swelling that had appeared. My temperature was still at 108 and my blood pressure was elevated. After they drew my blood they came back and said I had an infection that was so bad that they would have to admit me. At that time it had been about 4 hours since I ate so my sugar could have been around 600 plus should I have eaten however it was in the 300s. I was started on diabetic treatments with insulin shots immediately. I was very concern about my stay in the hospital because I was there for 9 days and my bill was over $30,000. It took them that long to stabilize my sugar levels. They finally found a splinter on the bottom of my foot and an ulcer between my toes about size of my pinky. This was an open ulcer with fluid draining from it. They sent me home on antibiotics, diabetic medication and blood pressure medications. I was told to clean my foot twice a day with iodine solution and to keep it elevated.

I went to an infectious disease Dr. for several weeks along with a foot specialist and primary Dr. No change was taken place in a positive direction however I had more fluid coming out and swelling that I have ever seen.

When I left the hospital I got a call form my friend Dan Gracia who briefly mentioned something about the inForce product based on the Coriolus Versicolor Mushroom. However I told Dan that I would do some research on it and I continued to do what the Dr. said by keeping my foot clean and staying off of it however it had gotten worst. Then about two weeks after the first call from Dan he called me again with his friend Jim Monde on the phone. At that time they where giving me a bunch of testimonies about this product.

The swelling and redness was getting so bad that it began to clime up my leg and it looked like I had Elephantitis and then other leg started to swell because I was depending on it so much. This entire time I had walked with a walker.

I was scheduled for a Dr.’s appointment the same week that Dan, Jim and I talked and I was not feeling good on the inside about what the Dr. might say because by this time I had been out of work for a couple of months and things where not looking good. I started thinking about my two brothers that are ready to loose there life. Both with cancer and the one with sever infections from a bad surgery. I went to the Dr. and as my gut was telling me he gave me the bad news that we would have to amputate my foot due to the infections. That was on the Thursday prior to the memorial weekend. After leaving the Dr.’s office I remembered what Dan and Jim had told me and started to pray about it. I then called Dan and gave him the bad news. Dan then told me that he would make a rush 24 hour order so that I would not have to spend the holiday weekend without the inForce product. The product arrived on the next day Friday and I immediately started to take them. I averaged about 7 pills a day until I saw the Dr. on Tuesday after the holiday. The Dr. stated to me that we are seeing a turn around here and healing of about 20-30% and it had only been 5 days since I started the product. After 7 days I saw more improvement and decided to increase my intake to 12 capsules a day. The following week I went back to the Dr. and he could not believe the rate of healing. He said it was improving so rapidly that the ulcer was healing up, the swelling was going down, the redness was subsiding and the fluid was slowing down. He also mentioned that at this pace I could be off of my crouches within a week to two weeks. I am scheduled to see him on September 26, 2010 I currently am still taking the product and have seen great improvement and also have notice my sugars’ are not bottoming out and blood pressure is staying normal. Because of the diabetes I have cataracts and bleeding behind the eyes. I have noticed improvement in my vision and I can’t wait to see what my eye Dr. has to say when I see him on November 12th to determine my surgery options for my eyes.

I am feeling much more energy, my skin is tightening and much smoother. I had a split finger nail from a fungus and it would not heal for over a year, it is now almost all healed. I don’t wake up in the middle of the night from sugar lows any more and my ringing in the ears has stopped. My mind is feeling sharper and I don’t feel like I have any forgetfulness.

This is a product that I recommend for anyone and everyone because the medical community is not telling us the entire truth about earths natural benefits. I want to thank the almighty, Dan Gracia, Jim Monde and inLife for introducing me to an amazing miracle product that I know will change the world.

Yours Truly

Love and blessings

Paulyah Ezekiel Kaufman

Allergy, Personal Testimonies

Allergies treated with Coriolus Versicolor also know as inForce, by inLife.

Hi. My name is Quintin and I use the inForce. It helps me get up earlier and fall asleep faster. It is very easy to swallow. I don’t have allergies anymore. The coriolus versicolor has helped me a lot.

Sincerely,
Quintin W – 11yrs old
Cleveland, Ohio

My name is Jennifer Welsh, I am Quintin’s mom. Quintin has suffered from severe allergies all year long for most of his childhood. Each year I spend a lot of time and money trying new products to help him and have had no success. Since using the inForce, I have noticed a 100% improvement with his allergies. Quintin has had more energy and has been able to wake up in the mornings for school on his own. The sneezing, coughing and stuffiness cleared up immediately. This has improved his quality of life so much that he is willing to take it without any nagging!

I would like to sincerely thank the co-founders of inLife for introducing this product.

Warm regards,

Jennifer Welsh
Regional Manager, inLife
Cleveland, Ohio

Medical Studies, PSP

The Influence of PSP on the Behavior and Growth of the Fetus in Rats

Zheng-de Zhang1, Zhen-bin Qian2, Lan-feng Zhou2 and Bin Xu1 1 Shanghai Institute of Materia Medica, Academia Sinica 2 Department of Toxicology, Shanghai Institute of Labour Health and Occupational Diseases

Abstract

PSP is a new immunomodulating agent prepared and developed by Professor Qing-yao Yang. In the present work its influence on the behavior and growth of fetus in 1st and 2nd generation of rats was investigated.

For behavior study the squirrel wheel test, rotating cylinder test and passive avoidance test were performed, PSP was administered orally at 60, 600 and 6000mg/kg. The results showed that no marked difference in the change of behavior was found between the control and treatment groups. The brain weights of two-generation rats had no marked change either.

For teratogeny test 110 rats were involved PSP was administered orally at 60, 600 and 6000mg/kg in different groups for 10 days. The conditions of the maternal body, embryonic survival and the function and growth of the fetuses were examined. After statistical tests no marked difference was observed in the control and treatment groups.

The data observed showed that PSP has no noticeable toxicity on fetus growth and on the behavioral performance of 1st and 2nd generation rats.

(Click here for detail)

in vitro, Medical Studies, PSK, PSP

PSP and PSK

Informatioin Office of the Research Institute of Fungi Shanghai Teachers University

PSP and PSK are the 2 products of Yun Zhi ratified by Chinese Ministry of Public Health and Japanese Ministry of Public Health respectively.

PSK was first manufactured by Kureha Chemical Industry Co. Ltd. The PS in PSK represents polysaccharide and K represents the first alphabet of the name of this Company. It was originally written as PS-K and was later changed to PSK. o; The commercial name of the product is Krestin.

PSP was prepared by Professor Qing-yao Yang. It is like PSK and is also a kind of compound polysaccharide. On the molecules of the polysaccharide, the small molecular protein (polypeptide) is connected. So it is called Yun Zhi Duo Tang Tai or Yun Zhi Tang Tai. The Tang Tai English names were originally glycopeptide, proteoglucan, glycosaminoglucan, etc. But the polysaccharide is all composed of N-acetyl-amino-hexose. But the polysaccharides of PSP and PSK are not composed of N-acetylamino-hexose. So it is not suitable to use the name. So the word “polysaccharopeptide” or “polysaccharide-peptide” is used and is abbreviated as PSP or Ps-p.

According to the different degrees of extraction, there are a series of PSP products. PSP directly extracted from the mycelia of Yun Zhi is called Yun Zhi Polysaccharide-peptide (Trade mark Qing Kang) and PSP polysacchardie-peptide (Landford). The former is sold on the market of Mainland China and the latter is according to the export specifications and is sold overseas. These 2 products are mainly used for tumorous patients.

The essence of the product is obtained by further isolation of the crude product. It is called Essence of Mushroom (Yun Zhi) (The sole distributor is Winsor Health Products Ltd., Hong Kong) used for healthy purposes.

Japan is quite specialized in the research of Yun Zhi. Besides PSK, Hirose, S. et al, (1970), Naruse S. and Takeda S. (in 1970) and Sugiura M. (in 1980) isolated two anticancerous components of the mycelia of Yun Zhi respectively. The former is called

ASTO and latter D–II. In addition, Ito H. et al (in 1974) extracted from the fermented mash of Yun Zhi an anti-tumor component which does not contain protein and it is called Coriolan. Its chemical components are glucans (by Hayashida S. et al, in 1992). But the above-mentioned three components still remain in the process of pharmacological research and was not used in clinical application.

Though PSP and PSK are all a kind of protein bound polysaccharide and are all extracted from the deep layer cultivated mycelia, yet they use the different strains, fermented medium and different extracted methods. Thus there is a certain difference between PSP and PSK. It is known that in the polysaccharide of PSP there is fucose, while there is no fucose in PSP, which contains arabinose and rhamnose; while there are no such ingredients in PSK. On the other hand, according to the pharmacological and clinical research, PSP has the definite effect of alleviating pain and increasing appetite, while there is no such report on PSK. Comparison of Two Characterisitics of PSP and PSK

Items compared

Similarities

Dissimilarities

Fungi

Yun Zhi Coriolus versicolor (Fr.) Quel

PSP: Cov-1 strain PSK: CM-101 strain

Drug produced

PSP: capsule PSK: loose package

Powder color

brown

PSP: brown PSK: dark brown

Raw materials

deep-layer cultivated mycelia (2N)

Fermentation technology

with glucose as the main carbon source (25oC, 3 days)

PSP: nitrogenous source: soya beancake powder PSK: nitrogenous source: peptone and yeast cake

Extract and isolate

obtained by immersion in hot water

PSP: isolate by alcoholic precipitation PSK: isolate by salting out with (NH4)2SO4

Medicinal ingredients

protein bound polysaccharide; average molecular wt. 1 x 105 Da the polysaccharide is formed from many monosaccahrides containing

PSP: polysaccharides contain arabinose and rhmanose, but no fucose PSK: polysaccharides do not

(Click here for detail)

Immunotherapy, in vitro, Medical Studies, PSP

Antitumor Effect of Polysaccharide Peptide of Coriolus versicolor (PSP) and its Mechanism

Jin-Xu Zhou, Xin-li Shen, Zu-ming Shen, Xiao-yu Li Department of Pharmacology I Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai 200031

Abstract

Polysaccharide peptide of Coriolus versicolor (PSP) is a new anti-tumor and immunomodulating drug. In this paper PSP showed direct inhibition on the cell proliferation of sarcoma 180 in vitro and inhibitory effect on the growth of murine sarcoma 180 in vivo. Owing to its direct cytotoxic effect was not strong, but at lower concentrations (10-20ug/ml) of PSP promoted the proliferation of T and pre-T cells of mouse thymus, increased the thymus weight, provided more number of lymphocytes, prevented the involuation of thymus in tumor bearing mice and antagonized the anti-tumor action of PSP combined with antilymphocyte serum. It is suggested the principal mechanism of anti-tumor activity of PSP was T-cell mediated cytotoxicity.

It has been known that some polysaccharides and polysaccharide peptide isolated from various natural sources, especially isolated from Basiodiomycetes have certain anti-tumor activities. The polysaccharide contained a main chain of an alpha and beta (1-4) glucan and a tightly bound 15-38% polypeptides (PSP) isolated from Coriolus versicolor (Fr) Quel. (Cov-1) by Professor Qing-yao Yang also exhibited antitumor action against mouse sarcoma 180 in vitro and in vivo. Recent experiments suggest three possible mechanism by which these PSP might act: (1) Potentiating of T-cell mediated cytotoxicity which killed more number of target-tumor cells. (2) Definite concentration of PSP produced direct cytotoxic activity in vitro. (3) Induction of tumorcidal macrophages killed more cancer cells. In this paper the antitumor action of PSP and its possible mechanism are reported

(Click here for detail)

Chemotherapy, Medical Studies, PSP

Clinical Experience in the Use of PSP

W.C. Xue and T.F. Liu Cancer Hospital, Shanghai Medical University

There is no really effective treatment for moderate and advanced stages of esophageal carcinoma. Although surgery for the earlier cases has been able to give a 5 years survival rate of 28.7%, such operable cases are relatively few. By far the greater majority are already in stage III to IV when first seen in the clinic, and radiotherapy alone in these cases has given a 5 years survival rate of only 8-14%. In order to improve treatment results, a variety of chemotherapeutic agents have been used in combination surgery, but so far no really effective drug has been found.

The drug PSP (polysaccharide-peptide of Coriolus versicolor) has been discovered and produced by Professor Qing-yao Yang of. It is a new anti-cancer and immuno-regulatory drug, similar to PSK (Krestin) but the effective component has been found to be larger than PSK. Experimental data has proved these properties of PSP, and in vitro as well as in vivo studies have all proved that PSP is superior to PSK. Of course, as is the case with all new drugs, the ultimate proof of its value will have to be shown by clinical application.


(Click here for detail)

Cancer, Medical Studies, Prostate Cancer

Cell growth and gene modulatory activities of Yunzhi (Windsor Wunxi) from mushroom Trametes versicolor in androgen-dependent and androgen-insensitive human prostate cancer cells. TC Hsieh, JM Wu. Department of Biochemistry and Molecular Biology, New York Medical College, Valhalla, NY 10595, USA. The incidence

The incidence of prostate cancer varies greatly throughout the world; it is highest in African-Americans and lowest in the Asian populations of China, India, and Japan. Geographical differences in both prevalence of latent prostate cancer and mortality have been postulated to be influenced by diverse tumor-promoting and protective factors, both environmental and dietary. Prostate cancer is a tumor with an extremely long latency; the pattern of prostate tumorigenesis, in terms of the display and sequence of appearance of particular molecular or biochemical features, or morphological changes, characterizing different stages of the carcinogenic process, is expected to be heterogeneous. Some insights into tumor heterogeneity and progression can be obtained from studies using cell lines, particularly those derived from different anatomical sites. The present study aims to investigate whether hormone-responsive LNCaP and androgen-refractory JCA-1, PC-3, and DU-145 prostate cancer cells are responsive to Yunzhi (YZ), a proprietary dietary supplement prepared from extracts of Trametes versicolor, also known as Coriolus versicolor (a mushroom consumed by Chinese for its purported health benefits), and to elucidate its mechanism of action. Ethanolic extracts (70%) of YZ significantly reduced LNCaP cell growth, down-regulated the levels of secreted PSA, but had less effects on the expression of intracellular PSA and did not affect levels of the androgen receptor. In androgen-unresponsive prostate cancer cells, YZ had a much less pronounced suppressive effect on proliferation of PC-3 and DU-145 cells, compared to LNCaP, and was inactive against JCA-1 cells. Western blot analyses show that the expression of Rb, a key regulatory protein in G1/S transition, and PCNA, integrally involved in mammalian cell DNA replication, were significantly reduced by treatment with YZ in PC-3 and DU-145 cells, respectively. In contradiction, none of these biochemical parameters were affected in JCA-1 cells under identical treatment conditions. Further analysis shows that YZ increased the levels of signal transducer and activator family of transcription factors STAT 1 and STAT 3 in JCA-1 and not LNCaP cells. The greater sensitivity of LNCaP cells to this polysaccharopeptide raises the possibility that YZ may be considered as an adjuvant therapy in the treatment of hormone responsive prostate cancer; additionally, it may have chemopreventive potential to restrict prostate tumorigenic progression from the hormone-dependent to the hormone-refractory state.

(Click here for detail)

Immunotherapy, Lymphocytes, Medical Studies, PSP

Evaluation of polysaccharopeptide effects against C6 glioma in combination with radiation.

XW Mao, LM Green, DS Gridley.

Department of Radiation Medicine (Radiobiology Program), Loma Linda University and Medical Center, Loma Linda, Calif. 92354, USA. xmao@dominion.llumc.edu

Long-term control of high-grade brain tumors is rarely achieved with current therapeutic regimens. The major goal of this study was to determine whether polysaccharopeptide (PSP), a crude polysaccharide peptide extract derived from Coriolus versicolor, a fungus, could enhance the effects of radiation against glioma cells in culture and in xenografted tumors in vivo. PSP significantly augmented radiation-induced damage to C6 rat glioma cells in vitro. Nude mice injected subcutaneously with the C6 cells were treated with PSP (injected intraperitoneally at 2 mg/injection) and radiation (2 Gy/fraction, 8 Gy in total) using three different time-dose protocols. Tumor volumes were consistently smaller in all treated groups compared to the non-treated tumor-bearing controls except in one group which received PSP prior to tumor implantation. The administration of radiation alone resulted in the slowest tumor progression, whereas PSP alone had no effect. Furthermore, PSP in combination with radiation treatment did not increase radiation efficacy. Natural killer cell, lymphocyte and granulocyte counts in blood and spleen were significantly higher in PSP-treated animals, demonstrating that PSP has protective effects on immunological function. Collectively, these results warrant further investigation to determine if PSP can be effectively utilized to upregulate immune responsiveness in case of neoplasia and other diseases in which immunosuppression is a prominent feature.

(Click here for detail)