Category Archives: PSP

Clinical Trials in Hong Kong Yunzhi-PSP

PSP has been shown to manifest immunomodulatory and anticancer properties in both pre-clinical experiments and clinical trials. It has been
shown to reduce the side effects of radiotherapy and chemotherapy and has been used as an adjunct medical modality to conventional cancer treatment. Experiments suggest that PSP can boost the immune system and alleviate the symptoms of chemotherapy.

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Clinical trail China phase 1

Polysaccharide-peptide (PSP) is a protein bound polysaccharides isolated from the COV-1 strain of Yunzhi (Coriolous versicolor mushroom) and made from modern alcohol extraction techniques. Each capsule contains 0.34 grams of PSP. Experimental in-vitro and in-vivo studies have shown PSP inhibits the proliferation of cancer cells including P338 leukemia cells, S 180 cells, Ehrlich ascites, and stomach and lung cancer cells. It also inhibits the growth of some tumors such as the lymphatic tumor of human skin tissue cells. In addition, PSP affects the immune system of mice by stimulating the production of ?\interferons, increasing the phagocytic index and metabolic rate of the reticuloendothilial system and by raising the HC 50 (median hemolytic dose), IgG and PFC (plaque forming cell) values. Human in-vivo experiments have also shown PSP can modulate the immune system by helping to prevent and partly eliminate the side effects of radiation and chemotherapeutic agents used by cancer patients.

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American Cancer Society

Coriolus versicolor is a mushroom used in traditional Asian herbal remedies (see Chinese Herbal Medicine). Two substances extracted from
the mushroom, polysaccharides K (PSK) and polysaccharides-peptide (PSP), are being studied as possible complementary cancer treatments.
Versicolor polysaccharides (VPS), another extract from the mushroom that is sold as a dietary supplement in the United States, is also being studied. A polysaccharide is a carbohydrate formed by a large number of sugar molecules.

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Antimetastatic Effects of PSK (Krestin), a Protein-bound Polysaccharide Obtained from Basidiomycetes: An Overview

PSK is currently used as an immuno therapeutic agent for gastric colorectal, and lung cancers in Japan. It has virtually no adverse effects, and it can be administered P. over a long term. Consequently, its use need not be limited to the treatment of Cancer, and, as our previous paper suggested, it should in the future prove valuable as a general chemopreventive agent and, as this review shows, as anti metastatic agent. The principal mechanisms of PSK may act as an inhibitor of the motility, invasion, and progression of tumor cells,  in addition to its role as an immunomodulator.

Antimetastatic Effects of PSK (Krestin), a Protein-bound Polysaccharide Obtained from Basidiomycetes: An Overview….. View more here:

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Yun zhi (Cloud Mushroom) PSP Extract- West Nile Virus and Yun zhi PSP

Yun zhi (Cloud Mushroom) PSP Extract

West Nile Virus and Yun zhi PSP

Now that it seems the worse of the SARS virus is over, a new virus is now being tested for its possible spread in the Lower Mainland. Last year, West Nile Virus was detected in the American states of Idaho, Montana, and Washington. It is expected this year that migrating birds will bring the virus into BC this summer.

West Nile Virus is a disease that is carried by birds, such as crows and ravens. However, it can be transmitted between birds and humans by mosquitoes. Now that warmer weather is approaching there is concern that the virus will spread. Protecting yourself against the spread of this infection can come in many forms. Among those are long sleeved shirts and pants, DEET-based mosquito repellent, and controlling the areas where mosquitoes can breed. The first line of defense however, is the one that everyone was born with – your immune system. The immune system is a vital part of any organism, and protects against invasion by viruses, bacteria, pollen, and other forms of infection. Without a healthy immune system, the body is unable to fight off infections, leading to symptoms that can include fever, body aches, and a rash.

Once a microorganism enters the body, it can multiply, and be toxic to the body’s biochemistry and other organs, or the multiplying microorganism can produce toxins to make the body ill. A healthy immune system will hopefully recognize the presence of these microorganisms as “foreign”, and immediately mobilize itself to produce white blood cells, antibodies, and other proteins to identify and destroy the invading foreign infectious agents.

For most people with healthy immune systems, these symptoms of West Nile Virus will usually disappear after about a week. However, one percent of those affected could experience more severe symptoms, such as confusion, headaches and paralysis, if their immune system cannot learn how to make the antibodies and other biochemicals that are needed to destroy the infecting virus. These people could then develop further complications such as meningitis or encephalitis. Ten percent of those people could die from the disease.

A weakened immune system can be caused by a combination of factors that can include stress, environment, aging, depression, and/or recent illness. Increasingly, many people are paying attention to what is called an alternative approach to health. It essentially involves taking care of the body, particularly the immune system, so that the body can take care of disease.

One approach can be the use of herbal remedies such as Yun zhi mushroom extract. It is only relatively recently that the world has become aware of the immuno-modulating properties of Yun zhi polysaccharides, but extensive research and clinical trials have shown that the regular use of natural Yun zhi can be effective in improving immune system function and response by increasing components of the immune system such as white blood cells, T-cells and B-cells.

Yun zhi polysaccharides have been used in the treatment of cancer and hepatitis in China and Japan for years. Patients who have used natural Yun zhi report increased strength and well-being, increased resistance to colds, flu, reduced fatigue and nausea, as well as regaining a normal appetite. Boosting the immune system through the regular use of natural Yun zhi could therefore provide increased protection against the threat of the West Nile Virus.

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The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor

Cheuk-Lun Lee, Xiaotong Yang, Jennifer Man-Fan Wan

Department of Zoology, Kardoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China

Received 24 December 2003; accepted 5 October 2004


Polysaccharopeptide (PSP) derives from the medicinal mushroom Coriolus versicolor is considered a biological response modifier with potential pharmaceutical applications. Significant literatures support the immune and anticancer functions of PSP; however, standardization is of big concern because variable biotechnological factors can affect both the chemical and biological properties of PSP. In this study, the extracts of PSP obtained at different days from the Coriolus versicolor culture were tested in vitro for their immune function on human normal peripheral blood mononuclear cells (PBMC) and cytotoxicity on the human leukemia Molt 4 cells. Over the 10-days culture period, both biomass and peptide/polysaccharide content were increased with time. The increase in proliferation index of PBMC and their production of interleukin 1 beta (IL-1_), tumor necrosis factor alpha (TNF-_) and gamma interferon (IFN-_) in the presence of PHA strengthens the correlation between culture duration and biological potency of PSP. The growth inhibition of the Molt 4 cells by PSP also depended on its maturity. Flow cytometry analysis on cell cycle and cell death (apoptosis) of Molt 4 cells indicated that the anticancer mechanism of PSP is related to its ability to induce S-phase cell arrest and apoptosis, respectively. Together, these results suggest that monitor the harvest duration is critical for the quality control of polysaccharopeptide in the biotechnological industry.

© 2005 Elsevier Inc. All rights reserved. Keywords: Coriolus versicolor; Polysaccharopeptide; Flow cytometry; High performance liquid chromatography; Peripheral blood mononuclear cells; Molt 4

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The Electrophretical Analysis of the Components of Polysaccharide-peptide (PSP)

Xiao-tong Yang and Yong Zhuang Shanghai Teachers University


The components of polysaccharide-peptide (PSP) are analysed by electrophoresis of cellulose acetate membrance and of polyacrylamide gel. The result shows that there are 2 kinds of glycoprotein and 2 kinds of polysaccharide. There are obvious differences between the numbers of components and the nature of electrophoresis of PSP and the polysaccharides of the fruit-bodies of Yun Zhi (CVP).

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Study on Anti-tumor Action of PSP

R.T. Chen, A.M. Zhou, B. Xu Department of Pharmacology I Shanghai Institute of Materia Medica, Academia Sinica


It has been reported that some polysaccharides possess antitumor action. PSP is a glycopeptide isolated from Coriolus versicolor by Yang et al. Its physiological properties have been investigated. In the present work we studied the antitumor action of PSP in vitro experiments.


PSP at the doses of 500 or 1000ug/ml produced inhibitory effect on P388 luekemia cells by 79-96%. At the dose of 1000 or 2000ug/ml PSP caused the inhibition of [3H]UR or [3H]TdR incorporation into RNA and DNA in Ehrlich ascites carcinoma cells was found to be the inhibition rate 50-80% or 27-47% respectively.

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The Influence of PSP on the Behavior and Growth of the Fetus in Rats.

Zheng-de Zhang1, Zhen-bin Qian2, Lan-feng Zhou2 and Bin Xu1 1 Shanghai Institute of Materia Medica, Academia Sinica 2 Department of Toxicology, Shanghai Institute of Labour Health and Occupational Diseases


PSP is a new immunomodulating agent prepared and developed by Professor Qing-yao Yang. In the present work its influence on the behavior and growth of fetus in 1st and 2nd generation of rats was investigated.

For behavior study the squirrel wheel test, rotating cylinder test and passive avoidance test were performed, PSP was administered orally at 60, 600 and 6000mg/kg. The results showed that no marked difference in the change of behavior was found between the control and treatment groups. The brain weights of two-generation rats had no marked change either.

For teratogeny test 110 rats were involved PSP was administered orally at 60, 600 and 6000mg/kg in different groups for 10 days. The conditions of the maternal body, embryonic survival and the function and growth of the fetuses were examined. After statistical tests no marked difference was observed in the control and treatment groups.

The data observed showed that PSP has no noticeable toxicity on fetus growth and on the behavioral performance of 1st and 2nd generation rats

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The Preliminary Appraisal of Polysaccharide Peptide (PSP) in Malignant and Non-malignant Diseases

Z.Y. Sun et al Shanghai Medical University


28 late cases of malignancy of all pathologically proven were evaluated. Among them are one case of melanoma, two cases of non-Hodgkin lymphoma (NHL), twenty cases of gastro-intestinal cancers, three cases of bronchogenic carcinoma, one case each of primary hepatocellular carcinoma and multiple peritoneal malignancies of unknown origin respectively.

Most of the cases had surgery, irradiation or anticancer chemotherapy in combination. PSP were taken orally in capsules, total dose ranged from 20g to more than 800g.

A case of malignant melanoma of back was operated for five times, she was operated for the first time in Jan 1982, lymphadenopathy of right iliac and inguired glands began, consequently wide dissections of the involved nodes were done. In August 1983, the disease metastatized to right chest wall and st. axillary glands and resected specimen showed one of three subseapular nodes and one of the eight axillary nodes were metastatic melanoma lesions, the estrogen receptor content of the specimen was 125F mol/mg. On September 10, the same year a total hysterectomy was done. She received 7 courses of CCNU, PCZ and VCR regime beginning from October 1983. Despite the stable condition of her disease, she had to give up further chemotherapy on account of distinct leucopenia. In April 1985, she was found to have GI bleeding, GI bladder, condition improved after Tamoxifen and symphomate treatments. Half year later melana reappeared and right hemicolectomy and partial resection of the jijunum were done. PSP was given postoperatively, and there was no chemotherapy for already more than 2 years. She is apparently well and can participate ordinary heavy work without difficulty.

Another case of multiple peritoneal metastasis of unknown origin was benefited by PSP also. She had mass of 5x5cm in RLQ of abdomen and quite massive ascites. Ager 100g

of PSP, ascites disappeared and the mass decreased to 3x3cm. White count went up to 5200 from 3800, lymphocytic mitosis increased from 28 to 36%.

Two cases of NHL were apparently benefited too by PSP in spite of the fact one each had received systemic chemotherapy and radiotherapy of waldegers ring respectively.

All the 28 cases except 2, the general conclitims of patients and appetite improved 2/3 of the cases showed an increase of white count of 1000 at least. Around half of the patients had an increased of function of cellular immunity. One case of liver cancer showed marked amelioration of abdominal pain 80 that he could abandon dolantin injection and one case of lung cancer had conspicuous decrease of the malignant pleural effusion.

Five cases of chronic gastrites and three cases of chronic active hepatitis showed remarkable improvement in symptoms and liver function test. HBsAg declined in two of three hepatitis patients.

So far no adverse drug reaction has been observed, there were no impairment of liver and renal functions after the long term administration of PSP even up to years.

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