Category Archives: Diseases

Allergy, Personal Testimonies, PSK

Jackie Ans Testimony

My name is Jackie Ans, I’m 44 years old, and have been battling several health problems for many years.  I also suffer from allergy and sinus conditions. After taking several medications for years and years my immune system was next to nothing. I also had been taking five different medications all at the same time to help fight my allergy and sinus issues.  Advair, Albuteral, Allegra-D, Astelin, and Mucinex.  I was constantly getting sick and had to use a lot of sick time at work due to these conditions!  If I worked overtime or did anything that required stamina for any period of time I paid for it.  I was constantly getting sinus and respiratory infections a long with the fighting the asthma.  I was at my wits end, and just felt miserable all the time.

One day at work, Mike Mackovjak, suggested that I should try this new product his wife was just getting involved in.  He stated that his nephew was having several issues with allergies and they started him on this new research drug called Inforce. It is suppose to help people with allergies and decreased immune issues.  So I talked to Paula regarding the research drug.  She explained to me how it came about, and what she believed it could do to help me out with my situation.  I told her “I have nothing to lose!  I’ll try it!”

I was a little hesitant to say anything to anyone about it because it was a brand new drug that nobody had heard anything about.  I do have several people in my family that have allergy and asthma problems. I knew my family would say, “That’s no good, there’s no proof that it helped anyone and you are just wasting your money!”  But, I had faith in Paula; I have known her for several years and she has helped me out in so many ways in the past.  I know she is a good hearted person and I totally trusted her opinion, so I signed up for it.

I was anxious to see what the results would be.  I wanted to be able to say to my family, “Look what this product has done for me!”  I wanted to see if this may be able to help them as well!

Within the first month of taking this drug, I was able to stop taking the Mucinex and the Astelin.  The next month I stopped the Allegra-D, and by the third month I stopped taking the Advair and Albuteral too! I felt fantastic!!! For the first time in I can’t remember when I did not have to use any sick time at work. My energy level was up and I didn’t have to deal with any of this!!!  My family was wondering why I started feeling better all of a sudden.  So I took that next step and told my family about taking the medicine. My Mom couldn’t believe it and was still a little bit worry about the whole thing. My step-father has been dealing with the same condition I have and I wanted him to start taking it.  I even gave them a bottle, so as to push them along.  After three weeks I asked my Mom if it was working.  She said she was still not sure about it and wanted their doctor to take a look at it.  To see what was actually in this bottle!  The doctor was amazed by the medication and gave my Mom the seal of approval! Right away he called his intern in and had her start doing some research on Inforce.  He is a doctor for senior citizens and thought that this may be able to help his other patients as well!  So you may be getting a big client!

I also have proof that this does help.  When I gave that bottle of medicine to my mother, I knew I was going to run out quicker, so I placed another order.  Unfortunately,  my timing was a little off and I did not get my order of new medicine in time.  The very first week I was without my medicine I got a severe sinus, respiratory, and mouth infection. I wound up on antibiotics and had to take three days off work because I could barely talk. For three weeks I have been battling this.  I started retaking inForce again about a week ago, but am still having some issues, so I called Paula yesterday to talk to her about it.  She advised me to double what I was taking and I should get better sooner.  It is nice to just be able to make a phone call to Paula about the product and she gladly answered my questions.  She is a great asset to your company and I’m proud to call her my friend!!!

Thank you,

Jackie Ans

Cancer, Immunotherapy, Leukemia, Medical Studies, PSP, Tumor

The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor

Cheuk-Lun Lee, Xiaotong Yang, Jennifer Man-Fan Wan

Department of Zoology, Kardoorie Biological Science Building, The University of Hong Kong, Pokfulam Road, Hong Kong SAR, China

Received 24 December 2003; accepted 5 October 2004

Abstract

Polysaccharopeptide (PSP) derives from the medicinal mushroom Coriolus versicolor is considered a biological response modifier with potential pharmaceutical applications. Significant literatures support the immune and anticancer functions of PSP; however, standardization is of big concern because variable biotechnological factors can affect both the chemical and biological properties of PSP. In this study, the extracts of PSP obtained at different days from the Coriolus versicolor culture were tested in vitro for their immune function on human normal peripheral blood mononuclear cells (PBMC) and cytotoxicity on the human leukemia Molt 4 cells. Over the 10-days culture period, both biomass and peptide/polysaccharide content were increased with time. The increase in proliferation index of PBMC and their production of interleukin 1 beta (IL-1_), tumor necrosis factor alpha (TNF-_) and gamma interferon (IFN-_) in the presence of PHA strengthens the correlation between culture duration and biological potency of PSP. The growth inhibition of the Molt 4 cells by PSP also depended on its maturity. Flow cytometry analysis on cell cycle and cell death (apoptosis) of Molt 4 cells indicated that the anticancer mechanism of PSP is related to its ability to induce S-phase cell arrest and apoptosis, respectively. Together, these results suggest that monitor the harvest duration is critical for the quality control of polysaccharopeptide in the biotechnological industry.

© 2005 Elsevier Inc. All rights reserved. Keywords: Coriolus versicolor; Polysaccharopeptide; Flow cytometry; High performance liquid chromatography; Peripheral blood mononuclear cells; Molt 4

(Click Here for Detail)

Cancer, Colon Cancer, Medical Studies, PSK

[Randomized controlled study on adjuvant immunochemotherapy with PSK in curatively resected colorectal cancer. The Cooperative Study Group of Surgical Adjuvant Immunochemotherapy for Cancer of Colon and Rectum]

[Article in Japanese]

Mitomi T, Tsuchiya S, Iijima N, Aso K, Suzuki K, Nishiyama K, Amano T, Takahashi T, Murayama N, Oka H, et al.

Dept. of Surgery II, Tokai University.

Abstract

To evaluate of adjuvant immunochemotherapy with PSK in curatively resected colorectal cancer, randomized controlled

study by 35 institutions in Kanagawa prefecture was conducted. From March 1985 till February 1987, 462 patients were

assigned one of two different regimens. 448 patients (97.0%) of them satisfied the eligibility criteria. Control group

received mitomycin C intravenously on the day and the day after the operations respectively followed by 5-FU orally over

for 6 months. PSK group received in addition to mitomycin C and 5-FU as in control group, PSK orally for over 3 years. By

February 1989, follow up studies of the patients after their operations had been carried out for two years to four years.

The disease free curve and the survival curve of PSK group were higher than those of control group, differences between

the two groups were statistically significant (Disease free curve: P = 0.0096, survival curve: p = 0.0391). From these

results, adjuvant immunochemotherapy with PSK was considered beneficial for curatively resected colorectal cancer.

PMID: 2500070 [PubMed – indexed for MEDLINE]

(Click Here for Detail)

Cytokines, Leukemia, Medical Studies, PSK, Tumor

Stimulation of interferon-gamma-induced human myelogenous leukemic cell differentiation by high molecular weight PSK subfraction.

Display Settings: Abstract

Anticancer Res. 1990 Jan-Feb;10(1):55-8.

Kim F, Sakagami H, Tanuma S, Konno K.

First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.

Abstract

PSK, a protein-bound polysaccharide extracted from the mycelia of Coriolus versicolor (Fr.) Quel, stimulated tumor

necrosis factor-induced cytotoxicity against mouse L-929 fibroblast. PSK also stimulated interferon-gamma-induced

differentiation of human myelogenous leukemic U-937 and THP-1 cells. The differentiated cells had higher proportions of

cells that expressed NBT-reducing activity and alpha-naphthyl acetate esterase activity. Among four PSK subfractions, the

highest molecular weight fraction (MW greater than 200 kD) had the most potent stimulating activity. This is the first report

regarding direct PSK modulation of cytokine action.

PMID: 2110432 [PubMed – indexed for MEDLINE]

(Click Here for Detail)

Cancer, Cytokines, Gastric Cancer, Medical Studies, PSK

Usefulness of immunomodulators for maturation of dendritic cells.

Display Settings: Abstract

Int J Oncol. 2004 Aug;25(2):453-9.

Ogihara T, Iinuma H, Okinaga K.

Department of Surgery, Teikyo University School of Medicine, Tokyo 173-0003, Japan.

Abstract

Biological response modifiers (BRMs) augment the cytotoxic activity of various effector cells by the induction of multiple

cytokines and suppression of immunosuppressive factors. BRMs are used extensively in adjuvant therapy for gastric

cancer in Japan. In dendritic cell (DC)-based vaccine therapy, the quality of DCs is important in inducing strong antitumor

immunity. A good manufacturing practice (GMP) grade agent for DCs maturation is desirable for safety. Here we report

the effects of two BRMs, OK432 and PSK, which are GMP grade agents for the functional maturation of DCs. OK432 and

PSK were examined in vitro, and compared with lipopolysaccharide (LPS) and a cytokine cocktail (IL-1beta, TNF-alpha,

IL-6 and PGE2). In the immunophenotypical analysis, the expression of CD80 and CD83 of DCs stimulated with OK-432

increased significantly compared with PSK and medium, and this up-regulation was the same as levels of DCs stimulated

with cytokine cocktail. DCs stimulated with OK-432 showed significantly higher production of IL-12 and Th1-type cytokines

(IL-2 and IFN-gamma) compared with DCs stimulated with LPS or cytokine cocktail. OK-432 stimulated DCs could induce

the significantly high level of cytotoxic T cell activity compared with PSK-stimulated or unstimulated DCs. These results

suggest that OK432 is a GMP-grade reagent that promotes functional maturation of DCs and could be applied in

DC-based vaccinations.

PMID: 15254744 [PubMed – indexed for MEDLINE]

(Click Here for Detail)

in vitro, Medical Studies, PSP, Tumor

Study on Anti-tumor Action of PSP

R.T. Chen, A.M. Zhou, B. Xu Department of Pharmacology I Shanghai Institute of Materia Medica, Academia Sinica

Abstract

It has been reported that some polysaccharides possess antitumor action. PSP is a glycopeptide isolated from Coriolus versicolor by Yang et al. Its physiological properties have been investigated. In the present work we studied the antitumor action of PSP in vitro experiments.

Summary

PSP at the doses of 500 or 1000ug/ml produced inhibitory effect on P388 luekemia cells by 79-96%. At the dose of 1000 or 2000ug/ml PSP caused the inhibition of [3H]UR or [3H]TdR incorporation into RNA and DNA in Ehrlich ascites carcinoma cells was found to be the inhibition rate 50-80% or 27-47% respectively.

(Click Here for Detail)

Cancer, Medical Studies, PSK, Tumor

Tumor growth promoting activity of an immunosuppressive substance and its modulation by protein-bound polysaccharide PSK

Masahiko Fujii, Takayoshi Fujii, Ken Saito, Norio Takahashi, Chikao Yoshikumi, Junji Kakuchi and Yoshio Kawai Biomedical Research Laboratories, Kureha Chemical Industry Co., Tokyo, Japan

The administration of PSK caused the mean tumor size to decrease slightly and the duration of survival to be prolonged in comparison with control group. In tumor-bearing animals treated with IS, the administration of PSK significantly decreased the tumor size and prolonged the survival rate.

Further studies are required to clarify the origin of IS and its function in the body. For that purpose, the experimental model used in the present study is useful. Serum levels of IS may serve as a parameter to monitor the efficacy of immunotherapy

cont. in link…

(Click Here for Detail)

Cancer, Chemotherapy, Medical Studies, Melanoma, PSP

The Preliminary Appraisal of Polysaccharide Peptide (PSP) in Malignant and Non-malignant Diseases

Z.Y. Sun et al Shanghai Medical University

Abstract

28 late cases of malignancy of all pathologically proven were evaluated. Among them are one case of melanoma, two cases of non-Hodgkin lymphoma (NHL), twenty cases of gastro-intestinal cancers, three cases of bronchogenic carcinoma, one case each of primary hepatocellular carcinoma and multiple peritoneal malignancies of unknown origin respectively.

Most of the cases had surgery, irradiation or anticancer chemotherapy in combination. PSP were taken orally in capsules, total dose ranged from 20g to more than 800g.

A case of malignant melanoma of back was operated for five times, she was operated for the first time in Jan 1982, lymphadenopathy of right iliac and inguired glands began, consequently wide dissections of the involved nodes were done. In August 1983, the disease metastatized to right chest wall and st. axillary glands and resected specimen showed one of three subseapular nodes and one of the eight axillary nodes were metastatic melanoma lesions, the estrogen receptor content of the specimen was 125F mol/mg. On September 10, the same year a total hysterectomy was done. She received 7 courses of CCNU, PCZ and VCR regime beginning from October 1983. Despite the stable condition of her disease, she had to give up further chemotherapy on account of distinct leucopenia. In April 1985, she was found to have GI bleeding, GI bladder, condition improved after Tamoxifen and symphomate treatments. Half year later melana reappeared and right hemicolectomy and partial resection of the jijunum were done. PSP was given postoperatively, and there was no chemotherapy for already more than 2 years. She is apparently well and can participate ordinary heavy work without difficulty.

Another case of multiple peritoneal metastasis of unknown origin was benefited by PSP also. She had mass of 5x5cm in RLQ of abdomen and quite massive ascites. Ager 100g

of PSP, ascites disappeared and the mass decreased to 3x3cm. White count went up to 5200 from 3800, lymphocytic mitosis increased from 28 to 36%.

Two cases of NHL were apparently benefited too by PSP in spite of the fact one each had received systemic chemotherapy and radiotherapy of waldegers ring respectively.

All the 28 cases except 2, the general conclitims of patients and appetite improved 2/3 of the cases showed an increase of white count of 1000 at least. Around half of the patients had an increased of function of cellular immunity. One case of liver cancer showed marked amelioration of abdominal pain 80 that he could abandon dolantin injection and one case of lung cancer had conspicuous decrease of the malignant pleural effusion.

Five cases of chronic gastrites and three cases of chronic active hepatitis showed remarkable improvement in symptoms and liver function test. HBsAg declined in two of three hepatitis patients.

So far no adverse drug reaction has been observed, there were no impairment of liver and renal functions after the long term administration of PSP even up to years.

(Click Here for Detail)

Medical Studies, PSK, Tumor

Suppression of in vivo tumor-induced angiogenesis by the protein-bound polysaccharide PSK.

U.S. National Library of Medicine

National Institutes of Health

In Vivo. 1994 Mar-Apr;8(2):247-50.

Kanoh T, Matsunaga K, Saito K, Fujii T.

Kureha Chemical Ind. Co., Ltd., Biomedical Research Laboratories, Tokyo, Japan.

Abstract

The anti-angiogenic effects of an antitumor protein-bound polysaccharide, PSK, obtained from cultured mycelia of Coriolus

versicolor in basidiomycetes were examined by the mouse dorsal air sac assay. PSK suppressed the mouse hepatoma

MH134-induced angiogenesis when assessed by morphological and biochemical examinations. This finding suggested that

the anti-metastatic effect of PSK is attributed to the suppression of tumor-induced angiogenesis.

PMID: 7522606 [PubMed – indexed for MEDLINE]

(Click Here for Detail)

Cancer, Leukemia, Medical Studies, PSK

Stimulation of human peripheral blood polymorphonuclear cell iodination by PSK subfractions.

Display Settings: Abstract

Anticancer Res. 1990 May-Jun;10(3):697-702.

Sakagami H, Kim F, Konno K.

First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.

Abstract

A protein-bound polysaccharide, PSK, extracted from the mycelium of Coriolus versicolor (Fr.) Quel, stimulated the

iodination (incorporation of radioactive iodine into an acid-insoluble fraction) of human peripheral blood polymorphonuclear

cells (PMN), human promyelocytic leukemic HL-60 cells and human myeloblastic leukemic ML-1 cells. In contrast, PSK did

not significantly increase the iodination of other cultured cell lines (U-937, THP-1, L-929, T98G, BALB 3T3). The PSK

stimulation of iodination of both PMN and HL-60 cells depended on incubation time and temperature, and was significantly

suppressed by the presence of myeloperoxidase inhibitors. Among various PSK subfractions, the highest molecular weight

fraction (MW greater than 200 kD), or the fraction precipitated at pH 4.0-4.5, stimulated the iodination most. In contrast,

natural and chemically modified glucans had little or no stimulation activity. The active PSK subfractions synergistically

enhanced TNF stimulation of PMN iodination. The data suggest the presence of some unique components in PSK which

directly stimulate the iodination of myeloperoxidase-positive cells.

PMID: 2369086 [PubMed – indexed for MEDLINE]

U.S. National Library of Medicine

National Institutes of Health

Publication Types, MeSH Terms, Substances

(Click Here for Detail)