Category Archives: Autoimmune Disease

Ulcerative Colitis saturated with alternative herbal treatment known as inForce Immune Builder.

Peter J. French says:

Personal Update:

As most of you now know from my testimony above, I quit smoking for the LAST time on June 1st 2010 and I used the inLife electronic cigarette to do so. I have to say right here, quitting with the e-cig was the easiest part. My big problems would start, (as I feared they would) a few weeks later. For more details about those problems, please see the video of my testimony above.)

In the mean time, thanks to the generosity of Craig Youngblood I was able to start taking inForce on June 3rd 2010, a full month before the company was able to start shipping and that allowed me to “saturate” myself with inForce right from the beginning.

Many people have been asking me not only “how I’m doing today” but what my regiment was as far as “dosages” were concerned. I’d like to take a few minutes to update all of you on both of these subjects and I hope the information will help you make healthful decisions for this upcoming, fabulous New Year, 2011!

After doing a tremendous amount of research online regarding ulcerative colitis, two conclusions kept surfacing over and over for me. One was that it is still unknown what actually causes U.C. and the other was that it appears the immune system is somehow connected. A little more research taught me that it’s an OVER active immune system that’s my worst enemy not an “under” active one! Huh… I thought… “That’s interesting!”

So, with my new understanding that the Coriolus Versicolor extract helps to “balance” the immune system with no known side effects associated with it, I decided to hit my system HARD for the first month and to accomplish this I took 5 capsules three times a day, every day, during June, (7.5 Bottles). I started bleeding (right on schedule) about three weeks into my regiment but this time something was different. First of all, it wasn’t a “lot” of blood as had always been the case in the past, (that was encouraging) and second of all, it wasn’t getting exponentially worse (which was also “always the case” in the past). In fact… by July 2nd, while still taking 5/5/5 my symptoms were actually clearing up, I was bleeding less! Needless to say, this was very encouraging to me.

For the second month (July) I dropped to 4 capsules 3 times a day every day. (4/4/4), (6 bottles) All the while my symptoms continued to improve.

For my third month (August) I dropped to 3 capsules 3 times a day (3/3/3) (4.5 bottles) and again, my symptoms continued to improve to the point that I’m practically symptom free by now.

By September I dropped to 3/3… (3 in the morning / 3 at night) and continued this dosage through to October 16th 2010, which was my very first 100% symptom free day! I continued 3/3 (3 Bottles) through October and then dropped to 2/2 for December. About mid December (the 15th I think) I decided to get real “fancy” and I dropped to 1/1 and low and behold on December 22nd (about a week later) I had a little bleeding. I thought crap!!! (no pun intended) and that day I popped 3 caps right away and then went back up to 2/2 where I am today.

Interestingly, the little bleeding I had was just one time and after “boosting” the dosage back up just a little, I have found my own personal “sweet spot” and continue to remain 100% symptom free to this day. Like Simon Lu’s Mom says, “Don’t waste it!”

To summarize all of this, I realize that I am dealing with a VERY serious condition and that most people are not afflicted with something this grim, medically speaking.

That said, what’s most interesting to me are the testimonies trickling in from friends and family who started their own inForce regiment using the same “saturate first” approach (for the first ninety days), then dropping down to 1/1 after that. They have seen all SORTS of conditions associated with the immune system just simply “melt” away! We’ve seen allergies disappear; psoriasis and rashes have cleared up; colds that didn’t “take hold;” and the list goes on.

What I’m suggesting to my family, friends and acquaintances when I first talk to them about inForce is what I’m now calling a 3-2-1 approach. 3 Bottles the first month, 2 bottles the second and 1 bottle a month after that. If a person is dealing with something more serious, common sense would dictate that you immediately see your doctor, hand him/her a bottle of inForce, (which we have done), follow all of their advice and unless you’re told otherwise by the doctor… go to 6-4-2.

We keep a couple extra bottles on hand for a quick “boost.” For example, if one of the kids feels a cold coming on, I’ll have them take 3/3 for that day and low and behold… the colds don’t take hold!

It’s all really quite remarkable yet so much simple common sense as well. We all have an immune system. Very few of us have a perfectly “balanced” immune system.
Stress, pollution, poor diet, current illnesses, all these things and more compromise our immune systems on a daily basis. inForce is simply “food” for our immune systems and mine was apparently starving to death!

I hope this follow up was helpful and encouraging to you. Never before have I experienced the “freedom” and good health that I’m experiencing at this time in my life. I’m smoke free and I’m not bleeding to death. It’s remarkable.

I thank Craig Youngblood, all the founders and especially Simon Lu for their faith and tenacity in bringing this product to market.

All the best to all of you in 2011!

Peter J, French

A Life long battle with arthritis, fibro, auto-immune disease, and scoliosis. A brighter future with inForce™ Immune Builder with PSK

Debórah Anne Kaufman says:
I was born with Scoliosis, Pediatric Fibromyalgia and a weakened auto-immune system. What that means is from infancy, until the age of 11 years old, I woke up in the morning to start my day, unable to open my eyes.

They were glued shut from an excess accumulation of mucus overnight, which would harden and interlock my eye-lashes thus, by dawn my eyes were sealed shut. My mother’s daily routine was to place hot, wet towels over my eyes to loosen the mucus and remove it without removing my eye-lashes as well. Once that was done and I could open my eyes to see, my mother would drive me to my doctors office, where I would have to sit at a machine, equivalent to a vacuum cleaner sucking excess mucus from each nostril. It was a painful, childhood experience which I will never forget.

Because of scoliosis, I had to wear a full metal brace from my neck to my tail bone with special shoes which forced me to walk on the heels of my feet.

Because of my weakened auto-immune system and multiple allergies, I had numerous weekly shots. Every year, as the seasons mildly changed, in Los Angeles, CA, from summer to fall I would become sick with a cold, flu and/or bronchitis throughout winter until the following year’s spring season.

For over 40 years, I suffered the flu, bronchitis and asthma during the fall and winter seasons. In spite of my health problems; since I was a hyperactive creative child, I participated in competitive and non-competitive sports, art, music and dance. I have had a full life of activity and the idea I might someday suffer from illness, disease and disorders never entered my conscious mind. That was until the day I lost my health and wellness. For me, I did not recognize and appreciate how fortunate I was until I lost what I had, something more important than any money or material one could buy. I lost my health and wellness.

For over 20 years in young adulthood, my body rapidly declined with illnesses, which generally do not occur until old adulthood. In my 30’s suddenly I was faced with severe illnesses which included adult fibromyalgia, scoliosis, osteoporosis, arthritis, degenerative disc disease and an auto-immune deficiency. These illnesses caused further disorders and diseases to my endocrine, nutritional, metabolic systems, blood and blood-forming organs (acute anemia, liver, kidney and gall bladder), nervous system and sense organs, circulatory and respiratory systems (asthma, bronchitis and acute pneumonia), digestive and genitourinary system (stomach, intestines and bladder), gynecological (infertility and early menopause), musculoskeletal systems and connective tissue (muscle spasms and atrophy, fractures, torn ligaments and tendons). My auto-immune system deficiency was so severe, I could not walk into a room with any ill-health individuals without contracting a cold, virus, flu or other ailments and would take months, often entire seasons to recover.

In 1990 at 28 years old, I lost the use of both arms and was in chronic pain 24/7 365 days of the year. All I could do was sit in the corner of a room and cry. After dozens upon dozens of doctor visits, the medical profession had no idea what was wrong with my body, nor how or what to do to restore my health. I was told this was how I would be for the rest of my life, there was NO CURE, and “the sooner I would be able to accept my lot in life the better.”

What was my response? To their surprise and disappointment, I responded, “NO WAY! I refuse to accept this as my lot in life. I will do and did anything necessary to find a way to improve my health and wellness! I refuse to give up inLife!”

Long-term chronic pain and illness not only affected my body physiologically, but also affected my mind – my memory, cognitive abilities, and sleep patterns suffered tremendously as well. In the end, as it is darkest before the dawn, I had become isolated, only venturing outside for doctor appointments, treatment(s) and physical therapy.

It has been a long journey, filled with challenges and tragedy, which I have learned to overcome with adversity. After 20 years of ill health, the last 10 of which were filled with chronic pain 24/7/365 days, I have survived 29 surgeries and almost died, to the best of my recollection, at least 15 times. Of the 29 surgeries, 27 were not to improve my health and wellness from illness, disease and disorders. Rather, they were emergency surgeries to repair the damage done to my body as a direct result of allergies and side-effects to pharmacological medications. These medications were given to me by numerous, well-intentioned, highly educated medical practitioners.

I met my inLife mentor, teacher and role-model, Brian B Bush, just a few months before my 29th surgery in December 2009. Barely able to walk, let alone function, Brian introduced me to the electronic cigarette, which I immediately purchased and utilized in preparation for surgery.

In March 2010, I underwent an anterior cervical discectomy. Just one hour following surgery, for the first time in 10 years, I awoke in recovery with NO continuous electrical current running down my spine radiating down both arms and legs. It was gone! Thanks to one of the top neurological surgeons in our country, I was pain free at last and given a new lease on life! I was ecstatic. I enjoyed my positive smoking alternative, the electronic cigarette, in the hospital with glee!

I was so elated to have my health restored, I had forgotten about my weakened auto-immune system and immediately began attending conferences and events to make up for lost time personally, professionally and financially. Within one week, I had attended two events with over 400 people in attendance. At some point, I had come into contact with someone who had ill health and immediately suffered the consequences. For 6 weeks I had a temperature of 103 and had to go to the emergency room on two occasions because I could not breathe. For 6 weeks, I was given strong antibiotics and large quantities of Ibuprophen, but could not break my fever. The hospital ran a full blood panel, which told them that my white blood count, auto-immune system and more, were significantly below normal. Hepatitis C, from blood transfusions over 20 years earlier had returned, my liver, kidney, intestines, stomach and other organs ceased to function properly and were shutting down.

That was when inLife introduced the nutritional supplement inForce™ Immune Builder with PSK (Polysaccharide Krestin) and PSP (Polysaccharide Peptide) from the Coriolus Versicolor mushroom. What is Coriolus Versicolor? It is a mushroom, which has been documented and utilized in Eastern Medicine, since the Ming Dynasty in the 1300s. Within 24 hours of taking inForce, my fever broke and I was able to breathe, get out of bed, bathe and eat! On the 8th day using inForce the hospital ran my blood levels again. My numbers went from significantly below normal to above normal. After using inForce for 30 days, the hospital ran my blood levels again. My numbers went from above normal to significantly above normal.

For the first time in 10 years, not only was I able to breathe, eat and bathe, but I was also able to sleep, regain memory and improve my cognitive abilities. I could walk without falling and fracturing bones. I could eat meat, vegetables and carbs in the same meal, without spending two days in bed rolling around in excruciating pain. I began applying to, and interviewing for, employment in my field of practice.

During this process, I have learned through experience something crucial to achieving optimal health and wellness. One of the problems involved when people have issues with health and wellness is that they stop doing what they did to restore their health and wellness. Through no intention of my own, I miscalculated my inForce intake and ran out of inForce for one week. In just 7 days, a dormant infection rapidly worsened and I had to undergo an emergency root canal. My neck suddenly looked like I had a golf ball on the inside of my throat, and my entire face swelled. The day of my emergency root canal my inForce shipment arrived. Within 24 hours back on inForce, the swelling and infection were gone, not dormant, but gone! Because of this, I continue to maintain a higher dosage of inForce on a daily regimen and make sure I order enough to not run out, EVER. It has now been 4 months since I began taking inForce.

For the first time in my life, at 48 years old, when summer turned to fall, I did not get sick. For the first time in my life, I am working with people who have illnesses of one kind or another, and I am not getting sick.

I have more energy and feel better than I have ever felt before. After years of chronic pain, fatigue and hopelessness, I did not think it would ever be possible to feel well again. Today, I have more joy, health, and wellness and hope than I ever imagined possible.

Because of my story, I have met hundreds, thousands possibly, of sick people with all kinds of health problems, many of which are life-threatening diseases, with no hope for a cure, no hope for the future, their children and grand-children.

Today, I get to work in the fields of medical and mental health. On a daily basis, I work on educating, teaching and role-modeling a life of fulfillment, health and wellness, a meaningful purpose – heart, mind, body, and soul. If my story can save just one person from suffering physically, psychologically, emotionally, and/or spiritually, then everything I have experienced has been well worth it. Through my experience, strength and hope, today, I get to spend my days helping people to improve their health and wellness, their families, their friends and community – to empower people to heal, find their meaningful purpose in life, and achieve their dreams and desires. Everything is about personal, professional, and financial freedom – so everyone can spend their days, their lives with their families, friends and community.

Today, I get to be a member of a special group of people, who believe as do I, in the infinite universe of abundance, the laws of attraction, to pay it forward. We are all connected – heart, mind, body, and soul – in an incredible world where we live and love inLife!

I invite you to stop working to live. Rather live and love to work inLife to help one another to achieve our fullest potential, meaningful purpose, personal, professional, and financial freedom … to do what we all wish to do more than anything else on earth – to live, love and spend TIME with our families, friends and loved ones. Join us – to be people helping people.

God bless you and welcome to becoming changed, to live and love inLife!

Your global friend,

Debórah Anne Kaufman

Using alternative herbal medicine instead of Chemotherapy.

Pamela Prizant says:
I had to go through chemotherapy in my early 30?s for breast cancer – if I had it to do over again I would only take the InForce and nothing else – in fact, if (God Forbid) I ever have the cancer again I will refuse taking horrible chemo and only take the InForce!! I totally believe in the InLife InForce Products and know that I am going to help people be saved from many auto-immune deficiency diseases!!

Dr. Mark Flannery Joins inLife LLC’s Board as Medical Advisor

Irvine, California inLife, LLC distributors of science-based Health & Wellness products, today announced Dr. Mark Flannery joins inLife’s Board as Medical Advisor. Dr. Flannery, who specializes in the treatment of autoimmune disorders and chronic illness using advanced nutritional therapies is a Diplomate of the College of Clinical Nutrition, a Diplomate of the Chiropractic Board of Clinical Nutrition, and a Certified Nutrition Specialist by the Certification Board for Nutrition Specialists, today joins inLife to bring awareness and enlightenment to the public of inLife’s new immunological nutritional supplement, inForce Immune Builder.

Dr. Flannery who assists his patients in achieving their optimal health by focusing on correcting the imbalances in their physiology says, “After much research and analization of inLife inForce Immune Builder’s main ingredient Coriolus versicolor, I am very proud to be part of a company that is on the verge of helping many, many people realize their true health potential. In reviewing the research and viewing many of the testimonials so far submitted to inLife I am convinced that inForce Immune Builder should be part of everyone’s daily nutritional routine.” Dr. Flannery will also soon be seen interviewed by Cristina Ferrare on inLife’s soon-to-be-viewed national infomercial about the science behind inForce Immune Builder.

About Coriolus Versicolor

The Coriolus Versicolor mushroom is one of the most widely studied supplements for its immune building properties. Worldwide, there have been over 400 animal and human studies on Coriolus versicolor with over a dozen placebo-based human trials conducted in the west. Traditionally, the Coriolus versicolor mushroom (known as Yun-zhi or cloud mushroom in China) has been used in China for several thousand years because of its immune boosting capabilities. In the 1980s, Dr. Yang conducted further studies and was able to isolate a much more potent strain using a different, alcohol-based extraction process. The result was Polysaccharopeptide or PSP. In the United States, top-ranked hospital and research institutes have reported that Coriolus versicolor helps boost the body’s immune systems with limited side effects and safety of daily oral doses for extended periods of time. In addition, Coriolus versicolor and its potential positive effects has been studied very closely by M.D. Anderson, University of Texas, Loma Linda University, Beth Israel Deaconess Medical Center (a teaching hospital of Harvard Medical School) , The University of San Diego, Sloan-Kettering Center (New York), and Bastyr University (Kenmore, Washington) just to name a few.

inLife Immune Builder with PSP and PSK

inLife offers Coriolus versicolor as a Daily Dietary Supplement in capsule form to help maintain and stimulate the body’s immune system. Coriolus versicolor and its high-potency extracts, PSK and PSP are among the most widely studied supplements for their immune building properties. One would be hard-pressed to find another immune boosting product that has had more research completed or positive comments associated with it. The amount of worldwide comments and studies is compelling. InForce Immune Builder is a proprietary blend of both Polysaccharide-K (PSK) and Polysaccharopeptide (PSP). Both offer much needed immune building assistance and they can be taken on a daily basis. The products are bottled in the United States in an FDA registered bottling facility that is CGMP compliant (Current Good Manufacturing Practices).

About inLife, LLC

Founded in 2007, inLife has been very successful in bringing to market products that have efficacies that are soundly based on scientific research. inLife products are now available in the U.S. as well as the U.K, Canada and Spain. For more information on inForce Immune Builder and the company, please review www.myinlife.com. For further details on inForce, journalists may contact Thomas Kiklas directly at 949-648-2525.

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Founded in 2007, inLife has been very successful in bringing to market products that have efficacies that are soundly based on scientific research. For further details on inForce, journalists may contact Thomas Kiklas directly at 949-648-2525.

Click here to download this press release.

Antitumor effects of residual tumor after cryoablation: the combined effect of residual tumor and a protein-bound polysaccharide on multiple liver metastases in a murine model

Masato Urano, Chihiro Tanaka, Yasuyuki Sugiyama, Kiichi Miya, and Shigetoyo Saji*

Cryoablation is a low-invasive surgical treatment for malignant tumors. It may induce an immunological response leading to the eradication of distant metastases or alternatively it might promote the growth of residual tumors. In this paper we confirm the occurrence of both phenomena and we describe the preventive effect of a protein-bound polysaccharide preparation. Metastatic liver tumors were produced in BALB/c mice by the intrasplenic inoculation of colon 26 cells and cryoablation was carried out usingliquid nitrogen ()170C) applied by a contact method. The value of combiningcryoablation with administration of the polysaccharide preparation in the prevention of growth of residual tumors was investigated. It was shown that the number of metastatic liver nodules and the size of the primary tumor at the site of inoculation in the spleen were significantly lower when the volume that was frozen was small. The production by splenocytes of the tumor necrosis factor TNF-a, interferon INF-c, and the interleukins IL-4 and IL-10 increased significantly after freezing and thawing of the tumor tissue. The polysaccharide treatment significantly reduced the production of IL-4 and IL-10 followingcryoablation; the production of TNF-a and INF-c was slightly promoted; the natural killer and cytotoxic T-cell activities of splenocytes were slightly enhanced. It was concluded that the polysaccharide preparation was beneficial by suppressing IL-4 and IL-10 production and might inhibit the growth of residual tumor that is sometimes induced by large-volume cryoablation.

Alternating immunotherapy of advanced gastric carcinoma: A randomized comparison of carbazilquinone and PSK to carbazilquinone in patients with curative gastric resection

A total of 103 patients with advanced gastric carcinoma were randomized after curative surgery to receive an alternate administration of carabzilquinone (CQ) and PSK (Krestin) or carbazilquinone alone.  Each course of therapies started 1 week after the surgical operation and therapy schedules consisted of 9 courses.  In each course of 6 weeks, CQ was administered on day 0, 8, and 15.  In combined immunochemotherapy group, PSK was given orally in 3-divided doses of 2g/m^2/day from the day of the third CQ administration for consecutive 4 weeks.  Estimated survival rate and cumulative survival curve were compared untilizing the data up to 7 years after the operation.  There was no overall significant difference in survival rates between the CQ plus PSK group and the CQ alone group, but a group of patients survived significantly longer when treated with the combination of CQ and PSK.  Neither in more advanced cases nor in cancers of early stages, the addition of PSK provided an additive effect.  The favorable result obtained in one subgroup treated with PSK, suggests that the use of this agent in treating gastric cancers should be carefully evaluated in terms of serosal infiltration and nodal metastasis.?

For full article click here.

Modulation of cytokine expression by traditional medicines: a review of herbal immunomodulators.

Spelman K, Burns J, Nichols D, Winters N, Ottersberg S, Tenborg M.

Clinical Division, Department of Herbal Medicine, Tai Sophia Institute, 7750 Montpelier Road, Laurel, MD 20723, USA. spelman123@earthlink.net.
Abstract

Modulation of cytokine secretion may offer novel approaches in the treatment of a variety of diseases. One strategy in the modulation of cytokine expression may be through the use of herbal medicines. A class of herbal medicines, known as immunomodulators, alters the activity of immune function through the dynamic regulation of informational molecules such as cytokines. This may offer an explanation of the effects of herbs on the immune system and other tissues. For this informal review, the authors surveyed the primary literature on medicinal plants and their effects on cytokine expression, taking special care to analyze research that utilized the multi-component extracts equivalent to or similar to what are used in traditional medicine, clinical phytotherapy, or in the marketplace.

METHODOLOGY: MEDLINE, EBSCO, and BIOSIS were used to identify research on botanical medicines, in whole or standardized form, that act on cytokine activity through different models, i.e., in vivo (human and animal), ex vivo, or in vitro.

RESULTS: Many medicinal plant extracts had effects on at least one cytokine. The most frequently studied cytokines were IL-1, IL-6, TNF, and IFN. Acalypha wilkesiana, Acanthopanax gracilistylus, Allium sativum, Ananus comosus, Cissampelos sympodialis, Coriolus versicolor, Curcuma longa, Echinacea purpurea, Grifola frondosa, Harpagophytum procumbens, Panax ginseng, Polygala tenuifolia, Poria cocos, Silybum marianum, Smilax glabra, Tinospora cordifolia, Uncaria tomentosa, and Withania somnifera demonstrate modulation of multiple cytokines.

CONCLUSION: The in vitro and in vivo research demonstrates that the reviewed botanical medicines modulate the secretion of multiple cytokines. The reported therapeutic success of these plants by traditional cultures and modern clinicians may be partially due to their effects on cytokines. Phytotherapy offers a potential therapeutic modality for the treatment of many differing conditions involving cytokines. Given the activity demonstrated by many of the reviewed herbal medicines and the increasing awareness of the broad-spectrum effects of cytokines on autoimmune conditions and chronic degenerative processes, further study of phytotherapy for cytokine-related diseases and syndromes is warranted.

PMID: 16813462 [PubMed – indexed for MEDLINE]Free Article

http://www.ncbi.nlm.nih.gov/pubmed/16813462

Induction of cell cycle changes and modulation of apoptogenic/anti-apoptotic and extracellular signaling regulatory protein expression by water extracts of I’m-Yunity (PSP).

Hsieh TC, Wu P, Park S, Wu JM.

Department of Biochemistry & Molecular Biology, New York Medical College, Valhalla, NY 10595, USA. Tze-Chen_Hsieh@nymc.edu

Abstract

BACKGROUND: I’m-Yunity (PSP) is a mushroom extract derived from deep-layer cultivated mycelia of the patented Cov-1 strain of Coriolus versicolor (CV), which contains as its main bioactive ingredient a family of polysaccharo-peptide with heterogeneous charge properties and molecular sizes. I’m-Yunity (PSP) is used as a dietary supplement by cancer patients and by individuals diagnosed with various chronic diseases. Laboratory studies have shown that I’m-Yunity (PSP) enhances immune functions and also modulates cellular responses to external challenges. Recently, I’m-Yunity (PSP) was also reported to exert potent anti-tumorigenic effects, evident by suppression of cell proliferation and induction of apoptosis in malignant cells. We investigate the mechanisms by which I’m-Yunity (PSP) elicits these effects.

METHODS: Human leukemia HL-60 and U-937 cells were incubated with increasing doses of aqueous extracts of I’m-Yunity (PSP). Control and treated cells were harvested at various times and analyzed for changes in: (1) cell proliferation and viability, (2) cell cycle phase transition, (3) induction of apoptosis, (4) expression of cell cycle, apoptogenic/anti-apoptotic, and extracellular regulatory proteins.

RESULTS: Aqueous extracts of I’m-Yunity (PSP) inhibited cell proliferation and induced apoptosis in HL-60 and U-937 cells, accompanied by a cell type-dependent disruption of the G1/S and G2/M phases of cell cycle progression. A more pronounced growth suppression was observed in treated HL-60 cells, which was correlated with time- and dose-dependent down regulation of the retinoblastoma protein Rb, diminution in the expression of anti-apoptotic proteins bcl-2 and survivin, increase in apoptogenic proteins bax and cytochrome c, and cleavage of poly(ADP-ribose) polymerase (PARP) from its native 112-kDa form to the 89-kDa truncated product. Moreover, I’m-Yunity (PSP)-treated HL-60 cells also showed a substantial decrease in p65 and to a lesser degree p50 forms of transcription factor NF-kappaB, which was accompanied by a reduction in the expression of cyclooxygenase 2 (COX2). I’m-Yunity (PSP) also elicited an increase in STAT1 (signal transducer and activator of transcription) and correspondingly, decrease in the expression of activated form of ERK (extracellular signal-regulated kinase).

CONCLUSION: Aqueous extracts of I’m-Yunity (PSP) induces cell cycle arrest and alterations in the expression of apoptogenic/anti-apoptotic and extracellular signaling regulatory proteins in human leukemia cells, the net result being suppression of proliferation and increase in apoptosis. These findings may contribute to the reported clinical and overall health effects of I’m-Yunity (PSP).

PMID: 16965632 [PubMed – indexed for MEDLINE]PMCID: PMC1574346Free PMC Article

http://www.ncbi.nlm.nih.gov/pubmed/16965632

Polysaccharopeptide enhances the anticancer activity of doxorubicin and etoposide on human breast cancer cells ZR-75-30.

Wan JM, Sit WH, Louie JC.

Food and Nutritional Science Division, School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, PR China. jmfwan@hkusua.hku.hk

Abstract

In search of natural bioactive microbial compounds with adjuvant properties, we have previously showed that the polysaccharopeptide (PSP), isolated from Chinese medicinal mushroom Coriolus versicolor, was able to enhance the cytotoxicity of certain S-phase targeted-drugs on human leukemic HL-60 cells via some cell-cycle and apoptotic-dependent pathways. The present study aimed to investigate whether the synergism of mechanisms of PSP with certain chemotherapeutic drugs also applies to human breast cancer. PSP treatment enhanced the cytotoxicity of doxorubicin (Doxo), etoposide (VP-16) but not cytarabine (Ara-C). Bivariate bromodeoxyuridine (BrdUrd)/DNA flow cytometry analysis estimated a longer DNA synthesis time (Ts) for the PSP treated cancerous cells suggesting that PSP enhanced the apoptotic effect of Doxo and VP-16 via creating an S-phase trap in the human breast cancer cell line ZR-75-30. The participation of PSP in the apoptotic machinery of the chemotherapeutic agents was further supported by a reduced ratio of protein expression of Bcl-xL/Bax of the cancer cells. This study provides further insight into the synergistic mechanisms of PSP and supports the hypothesis that the anticancer potentials of PSP is not limited to leukemia but may also be used as an adjuvant therapy for breast cancers.

PMID: 18292947 [PubMed – indexed for MEDLINE]

http://www.ncbi.nlm.nih.gov/pubmed/18292947