Stimulation of human peripheral blood polymorphonuclear cell iodination by PSK subfractions.

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Anticancer Res. 1990 May-Jun;10(3):697-702.

Sakagami H, Kim F, Konno K.

First Department of Biochemistry, School of Medicine, Showa University, Tokyo, Japan.

Abstract

A protein-bound polysaccharide, PSK, extracted from the mycelium of Coriolus versicolor (Fr.) Quel, stimulated the

iodination (incorporation of radioactive iodine into an acid-insoluble fraction) of human peripheral blood polymorphonuclear

cells (PMN), human promyelocytic leukemic HL-60 cells and human myeloblastic leukemic ML-1 cells. In contrast, PSK did

not significantly increase the iodination of other cultured cell lines (U-937, THP-1, L-929, T98G, BALB 3T3). The PSK

stimulation of iodination of both PMN and HL-60 cells depended on incubation time and temperature, and was significantly

suppressed by the presence of myeloperoxidase inhibitors. Among various PSK subfractions, the highest molecular weight

fraction (MW greater than 200 kD), or the fraction precipitated at pH 4.0-4.5, stimulated the iodination most. In contrast,

natural and chemically modified glucans had little or no stimulation activity. The active PSK subfractions synergistically

enhanced TNF stimulation of PMN iodination. The data suggest the presence of some unique components in PSK which

directly stimulate the iodination of myeloperoxidase-positive cells.

PMID: 2369086 [PubMed – indexed for MEDLINE]

U.S. National Library of Medicine

National Institutes of Health

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