Displaying posts published in

November 2010

Chemical structure of antitumor polysaccharide, coriolan, produced by Coriolus versicolor.

Miyazaki T, Yadomae T, Sugiura M, Ito H, Fujii K.

PMID: 4430020 [PubMed – indexed for MEDLINE][…]

[Antineoplastic activities of Basidiomycetes; antineoplastic activities of polysaccharides produced by Coriolus versicolor Fries]

[Article in Japanese]

Fujii K, Ito H, Naruse S.

PMID: 4477091 [PubMed – indexed for MEDLINE][…]

[Studies on antitumor activities of Basidiomycetes-antitumor activity of polysaccharides and sex factors]

We have already reported antitumor activities of fungal and bacterial polysaccharides on mice. In the present experiment, the influence of the sex on antitumor effects on such material from Grifola umbellata, Coriolus versicolor Fries or Sargassum thumbergii and the immunity of mice against tumor were investigated. The growth velocities of Sarcoma 180, Ehrlich solid carcinoma, Pulmonary tumor 7423 and MF-sarcoma bearing mice both without treatment and those treated with polysaccharides were more rapid in males than in females. The regression rates in mice with the above tumors were higher in females than in males. However, a few DS Mie mice with Sarcoma 180 and A/Jax Mie mice with Ehrlich solid carcinoma regressed spontaneously. The growth velocity of Shionogi carcinoma 42 was not influenced by the sex. On other hand, both males and females which had experienced a regression of ascites tumor after the administration of polysaccharides rejected the re-implanted Ehrlich ascites carcinoma, Sarcoma 180, NF-sarcomma and Shionogi carcinoma 42. These results suggest that a strong ehancement of immune response occurs in the tumor implanted in the host animal by the administration of polysacchrides. The combination of X-ray irradiation Ehrlich ascites cells and polysacchrides strengthens the antitumor effect of NF-sarcoma and Shionogi carcinoma 42. Peritoneal exudate cells and lymphocytes were compared between the male and female mice after being treated with ATSO and P.GU-1. Such cells were present to a much greater extent in females.[…]

[Structural studies on the anti-tumor active polysaccharides from Coriolus versicolor (Basidiomycetes). II. Structures of beta-D-glucan moieties of fractionated polysaccharides (author’s transl)]

[Article in Japanese]

Hirase S, Nakai S, Akatsu T, Kobayashi A, Oohara M.[…]

Requirement for a growth substrate during lignin decomposition by two wood-rotting fungi.

Decomposition of C-labeled lignin to CO(2) by the lignin-decomposing fungi Phanerochaete chrysosporium and Coriolus versicolor required a growth substrate such as cellulose or glucose. Growth with lignin as sole carbon addition to an otherwise complete medium was negligible.[…]

Blastoid transformation of human lymphocytes cultured with protein-bound polysaccharide preparation, PS-K, in vitro.

The protein-bound polysaccharide preparation, PS-K, isolated from a mushroom, Coriolus versicolor, was found to stimulate human lymphocytes and induce them into blastogenesis in vitro. This stimulatory effect seemed to be nonspecific since lymphocytes from cord blood of newborn babies were also stimulated by PS-K. The highest lymphocyte blastogenesis by PS-K was observed after 5 days in culture.[…]

[Effect of ATSO from Coriolus versicolor on immune response (author’s transl)]

[Effect of ATSO from Coriolus versicolor on immune response (author’s transl)][…]

P.M.R. studies on the antitumour polysaccharide isolated from Coriolus versicolor.

Inoue Y, Chûjô R.

PMID: 902267 [PubMed – indexed for MEDLINE][…]

Enhanced induction of immune resistance by concanavalin A-bound L1210 vaccine and an immunopotentiator prepared from Coriolus versicolor.

Combined administration of a vaccine consisting of a small number (2 X 10(6)) of L1210 murine leukemic cells treated with glutaraldehyde and concanavalin A and a protein-bound polysaccharide preparation of Coriolus versicolor induced synergistic resistance to L1210 leukemia in BALB/c X DBA/2CrF1 mice. This effect was dependent on the dose and timing of the administration of the protein-bound polysaccharide preparation, being most effective at the time of or 1 day after the second vaccination. Induced resistance was not cross-reactive with P388 murine leukemia, indicating specificity of resistance. This immunopotentiation by the protein-bound polysaccharide did not occur when L1210 cells treated with glutaraldehyde, but not with concanavalin A, were used as a vaccine.[…]

Induction of laccase in Basidiomycetes: the laccase-coding messenger.

Formation of the mRNA specific for the inducible forms of laccase was evidenced in Coriolus versicolor, Pleurotus ostreatus and Pholiota mutabilis. The half-life time of these mRNAs in the fungi species studied were, respectively, 30, 37 and 24 min. Molecular weight of the newly synthesized mRNA in Pleurotus ostreatus was about 4.5X10(5), consistently with the size of the inducible laccase protein. The polysome obtained from the ferulic acid-treated mycelium, synthesized in vitro a polypeptide with the electrophoretic mobility similar to that of laccase.[…]