CLONING OF SEQUENCES INDUCED AND SUPPRESSED BY ADMINISTRATION OF PSK, ANTITUMOR PROTEIN-BOUND POLYSACCHARIDE

Kunitaka Hirose, Michinori Hakozaki, Kenichi Matsunaga, Chikao Yoshikumi, Tetsuya Hotta, Masaaki Yanagisawa, Mikio Yamamoto, Hideya Endo.

To elucidate the effects of PSK, a protein-bound polysaccharide from Coriolus versicolor, on gene expression in tumor cells, we prepared cDNA clone libraries from PSKtreated and untreated cells of a rat ascites hepatoma line, AH66, which was previously shown to be susceptible to the antitumor action of this compound. Two PSK-induced and one suppressed cDNA clones were selected from these libraries by using a differential colony hybridization and RNA blot hybridization. PSK was thus shown to have a direct effect on the transcription and consequently on the translation of tumor cells.

Full article: http://mushroomstudies.co/wp-content/uploads/2010/09/Cloning-of-sequences-induced-and-suppressed-by-administration-of-PSK-antitumor-protein-bound-polysaccharide.pdf

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